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  • Bruno Vellutini 14:06 on 2013/02/12 Permalink
    Tags: sequencing, ,   

    Re-sequencing Terebratalia Wnt7 


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    Sequences of Wnt7 were crap. Re-doing them including SP6 primer.

    ID GENE COLONY NEW ID PRIMER
    BV214 Wnt7 1 BV220 T7
    BV215 Wnt7 2 BV221 T7
    BV216 Wnt7 4 BV222 T7
    BV214 Wnt7 1 BV223 SP6
    BV215 Wnt7 2 BV224 SP6
    BV216 Wnt7 4 BV225 SP6

    13/02/13

    Nice sequences now! Beginning probe PCR.

     
  • Bruno Vellutini 17:20 on 2012/04/27 Permalink
    Tags: , , , , , sequencing, , ,   


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    Sequencing for Terebratalia segmentation genes cloned by Katrine/Andi. Just ran a sequencing PCR and sent the products to the sequencing facility.

    03/05/12

    Quickly checked the sequences today and they look bad. The only genes properly cloned were Patched and Netrin. The single Netrin sequence looks ok while the 2 Patched ones are a bit trashed.

     
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