Tagged: neuropeptides Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 15:12 on 2013/08/31 Permalink
    Tags: , neuropeptides   

    Extraction of Membranipora neuropeptides 

    Collected thousands of cyphonautes larvae from early (3d) to older than a week into a plastic syringe with 5 µm mesh immersed in FSW. Once all the larvae were concentrated I immersed the syringe in mqH2O and pipetted the larvae to a clean extraction tube with 300 µL of extraction buffer.

    I used the screwdriver with a pistil to intensively smash the larvae and completed the volume of the tube with extraction buffer. Tube was vortexed for 2 minutes and spinned down for 20 min at 4 °C. Supernatant was transferred to a clean tube and kept at -20 °C.

    I noticed that the larvae were not completely into pieces (although they were crushed), but I did what I could do with the screwdriver.

     
  • Bruno Vellutini 13:04 on 2013/06/19 Permalink
    Tags: , neuropeptides   

    Isodiametra pulchra neuropeptide extraction 

    Extracted neuropeptides off the acoel Isodiametra pulchra using Gaspar Jekely protocol. Instead of using a mesh I gave a quick wash on distilled H2O, spin down the worms, then added 250 µL of extraction buffer and drilled them. After I added 350 µL more and continued with the protocol (20 min spin at 4°C). After spinning there was remnants of worms in the bottom, so I just took the clear supernatant and transferred to a new tube.

     
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