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  • Bruno Vellutini 09:59 on 2012/06/01 Permalink
    Tags: , muscle   


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    Got a cod sample and fixed it in 4% PFA at 4 °C overnight.

    02/06

    Stained the smaller samples with Phallacidin for 2h and prepared the slides for the confocal. It was harder then the jellyfish because the fibers are packed in bundles and do not spread flat in the slide. Tried disrupting them in various way and found out that the staining was not so good in the inner fibers. Took enough pictures anyway.

     
  • Bruno Vellutini 18:00 on 2012/05/29 Permalink
    Tags: , muscle   


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    We collected a jellyfish for muscle Phallacidin staining for Andi’s News & Views article. I cut small pieces of the marginal region of the umbrella and followed the standard fixation protocol for confocal stainings. I also cut larger portions of the same region and fixed in 4% PFA overnight at 4 °C.

    30/05/12

    Washed the larger portions in PBS+Triton-X during the whole day and transferred to PBS at 4 °C. I stained one well with Phallacidin and looked in the fluorescent lamp to find the muscle layer. It is an opaque sheath of tissue below the mesoglea. The staining worked.

    31/05/12

    Dissected the muscle layer better and mounted the slide. The tissue is well spread and staining looks ok. Confocal tomorrow.

    01/06/12

    Confocal time.

     
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