Tagged: Lineus viridis Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 17:10 on 2014/04/28 Permalink
    Tags: Lineus viridis   

    Fixed 20d Lineus. Larval epidermis is clearly visible in smaller embryos. More than half of the embryos had already hatched from the larval epidermis, specially fat embryos. I have relaxed the worms before fixation doing 30 min of MgCl2 in the cold room. Fixed in 4% FA diluted in magnesium chloride.

     
  • Bruno Vellutini 14:15 on 2014/04/15 Permalink
    Tags: Lineus viridis   

    Looking again at the wild caught cocoons from Lineus viridis I realized that, in fact, the eggs were not advanced blastula, but zygotes! Since then they haven’ t started cleaving! The egg mass that was laid on the day that we put then in the cold room had only one embryo at early cleavage, the rest were still 1 cell.

    So, we don’t know the actual fertilization rate of wild cocoons! I put them back at 14 °C to see what happens. Hopefully they start cleaving.

     
  • Bruno Vellutini 12:12 on 2014/04/14 Permalink
    Tags: Lineus viridis   

    Moved adult Lineus from the cold room back to the incubator at 14 °C to see if they start laying eggs.

     
  • Bruno Vellutini 19:04 on 2014/04/12 Permalink
    Tags: Lineus viridis   

    Fixed 7d Lineus viridis for in situ only. Chema is fixing 5d.

     
  • Bruno Vellutini 18:59 on 2014/04/09 Permalink
    Tags: Lineus viridis   

    The girls went collecting Lineus viridis and found 4 cocoons in the rocks. They seem to be from L. viridis and not from the other similar species due to the amount of embryos per capsule. L. viridis has many, around 10-15, while the other species captured last year laid cocoons with 2-3 embryos per capsule.

    Surprinsingly, all embryos withing each capsule seem to be healthy and developing! Fertilization in the field is much more efficient in the field. Our first guess is that the temperature is the problem. Water temperature is around 6 °C and we set our incubator to 14 °C to get the summer reproductive feeling and also because that is the temperature of the original description by von Döhren (10.1007/s10152-011-0266-z).

    Our strategy then was to leave the incubator at 14 °C to keep the egg masses at the original temperature because we already know the developmental staging. We then moved the adult worms to the cold room where the temperature is 5 °C. There 2 tanks, old worms from the first collection and a few new worms from the last collection.

    The goal is to wait them to lay a cocoon at this temperature and check for the fertilization rate. It might be higher!

    Bibliography

     
  • Bruno Vellutini 16:21 on 2014/04/04 Permalink
    Tags: Lineus viridis   

    Dissected and fixed 4d old embryos, around 60 good ones and plenty of weird.

     
  • Bruno Vellutini 15:59 on 2014/04/03 Permalink
    Tags: Lineus viridis   

    Dissected and fixed 3d old. Approximately 200 embryos.

     
  • Bruno Vellutini 15:20 on 2014/04/01 Permalink
    Tags: dmh1, , Lineus viridis,   

    First inhibitor experiment with L. viridis 

    Auxane and me set an inhibitor experiment with DMH1 and U0126 with early cleaving embryos from Lineus viridis. We dissociated the packets from the gelatinous layer and incubated with the following treatments.

    DMH1 1µM DMH1 5µM U0126 1µM U0126 5µM
    DMH1 10µM DMSO (1%) U0126 10µM FSW

    02/04/14

    No apparent difference between the experimental samples. Similar amounts of normal/weird/non-cleaving embryos.

     
  • Bruno Vellutini 08:34 on 2014/03/31 Permalink
    Tags: Lineus viridis   

    Lineus viridis laid the first three egg masses in the lab from this year’s first collection.

    01/04/2014

    3 more egg masses.

     
  • Bruno Vellutini 16:43 on 2014/03/13 Permalink
    Tags: , Lineus viridis   

    Nemertine collection at Aina’s house. Everything went well and we collected many Lineus viridis. Let’s see when they begin to lay egg cases.

     
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