Tagged: Isodiametra_pulchra Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 13:04 on 2013/06/19 Permalink
    Tags: Isodiametra_pulchra,   

    Isodiametra pulchra neuropeptide extraction 

    Extracted neuropeptides off the acoel Isodiametra pulchra using Gaspar Jekely protocol. Instead of using a mesh I gave a quick wash on distilled H2O, spin down the worms, then added 250 µL of extraction buffer and drilled them. After I added 350 µL more and continued with the protocol (20 min spin at 4°C). After spinning there was remnants of worms in the bottom, so I just took the clear supernatant and transferred to a new tube.

     
  • Bruno Vellutini 15:54 on 2013/03/24 Permalink
    Tags: Isodiametra_pulchra,   

    Starting a Isodiametra RNAseq CLC assembly with the following parameters:

    Automatic word size = ON
    Automatic bubble size = ON
    Minimum contig length = 170
    Auto-detect paired distances = ON
    Perform scaffolding = OFF
    
    Map reads back = ON
      mismatch = 2
      insertion = 3
      deletion = 3
      length = 0.5
      similarity = 0.8
    Colorspace alignment = ON
    Global alignment = OFF
    Update contigs = ON
    Create list of un-mapped = OFF
    
    Create report = ON
    Result handling = Save
    Make log = ON
    

    It started at 16h and finished 01h00. ~9h running with 24 cores.

     
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