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  • Bruno Vellutini 16:28 on 2013/08/02 Permalink
    Tags: , , , gustavus, , , , ,   

    Meara in situ for RNA binding proteins 


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    Starting in situ with mixed stages of Meara. 15 min protk.

    ago a ago b boule bruno a
    gus a mago orb2 staufen

    03/08/13

    Added probes.

    05/08/13

    First day of washes. Some small juveniles have ripped tissues. I guess the 15min protk is too much for them, the rest looks ok.

    06/08/13

    Regular 5 PBT and 5 PTw antibody washes. Kept rocking in cold room.

    07/08/13

    Developing day. Stopped argonaute after ~3h, they were becoming too dark. Bruno, Staufen are clearly there. Boule is weaker, but something. The rest there is nothing. Putting new AP in cold room.

    08/08/13

    In addition to Argonaute I also stopped Bruno, Staufen and Orb2 before going to the collection trip. The rest are developing overnight a bit more (also included Orb2 to develop overnight.

     
  • Bruno Vellutini 11:26 on 2013/05/21 Permalink
    Tags: , , , gustavus, , , , ,   

    Probe synthesis for Meara RNA binding proteins 


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    Began the probe PCR for RNA binding proteins in Meara. Finished by May 24th.

    Gene ID ENZYME ng/┬ÁL
    Ms gus a BV226 T7 3409
    Ms boule BV229 SP6 501
    Ms staufen BV233 SP6 1141
    Ms ago a BV237 SP6 563
    Ms ago b BV238 SP6 1236
    Ms bru a BV242 SP6 1226
    Ms mago nashi BV244 SP6 336
    Ms orb2 BV247 T7 473

    Ms RNA binding

     
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