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  • Bruno Vellutini 18:28 on 2012/08/27 Permalink
    Tags: foxg, , , nk6, , Prostheceraeus vittatus, ,   

    Probe synthesis for Terebratalia, Membranipora and Prostheceraeus 


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    Started a probe pcr with the following minipreps:

    Tt gli BV157 T7 3312,97
    Tt smo BV153 SP6 1154,37
    Tt nk6 BV173 SP6 1179,23
    Mm syt1a BV86 SP6 719,42
    Mm syt1b BV89 T7 942,87
    Pv syt1b BV189 T7 921,73
    Pv foxg BV191 SP6 1464,56
    Pv syt4a BV194 T7 1354,31

     
  • Bruno Vellutini 23:50 on 2012/04/01 Permalink
    Tags: , foxg, , , , ,   

    More Membranipora genes: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b 


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    Set a PCR to clone: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b

    02/04/12

    Bands with expected lengths. Purified DNA, set ligation reaction for 1h45 at RT, heatshock transformation and plated colonies with Xgal.

    03/04/12

    Plates were wetty, so colonies did not spread well… some were forming strings; but enough to pick. Picked white colonies for colony PCR:

    09/04/12

    Sequencing PCR and dropped in the SeqLab.

    BV75 Membranipora membranacea Mex3 T7
    BV76 Membranipora membranacea Fez T7
    BV77 Membranipora membranacea Fez T7
    BV78 Membranipora membranacea Fez T7
    BV79 Membranipora membranacea PL10 T7
    BV80 Membranipora membranacea PL10 T7
    BV81 Membranipora membranacea PL10 T7
    BV82 Membranipora membranacea FoxG T7
    BV83 Membranipora membranacea FoxG T7
    BV84 Membranipora membranacea FoxG T7
    BV85 Membranipora membranacea Syt1a T7
    BV86 Membranipora membranacea Syt1a T7
    BV87 Membranipora membranacea Syt1a T7
    BV88 Membranipora membranacea Syt1b T7
    BV89 Membranipora membranacea Syt1b T7
    BV90 Membranipora membranacea Syt1b T7

     
  • Bruno Vellutini 11:56 on 2012/03/22 Permalink
    Tags: , foxg, , , otp, , ,   


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    Membranipora primers have arrived: Mm Syt1a, Syt1b, NK2.1, FoxG, Otp, PL10, Fez.

     
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