PhD notebook

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  Bruno Vellutini 23:50 on 2012/04/01 Permalink
  Tags: fez, foxg ( 3 ), Membranipora membranacea ( 140 ), mex ( 5 ), pcr ( 37 ), pl10 ( 6 ), synaptotagmin ( 5 )   

  More Membranipora genes: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b 

  Set a PCR to clone: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b

  02/04/12

  

  Bands with expected lengths. Purified DNA, set ligation reaction for 1h45 at RT, heatshock transformation and plated colonies with Xgal.

  03/04/12

  Plates were wetty, so colonies did not spread well… some were forming strings; but enough to pick. Picked white colonies for colony PCR:

  

  09/04/12

  Sequencing PCR and dropped in the SeqLab.

  BV75	Membranipora membranacea	Mex3	T7
  BV76	Membranipora membranacea	Fez	T7
  BV77	Membranipora membranacea	Fez	T7
  BV78	Membranipora membranacea	Fez	T7
  BV79	Membranipora membranacea	PL10	T7
  BV80	Membranipora membranacea	PL10	T7
  BV81	Membranipora membranacea	PL10	T7
  BV82	Membranipora membranacea	FoxG	T7
  BV83	Membranipora membranacea	FoxG	T7
  BV84	Membranipora membranacea	FoxG	T7
  BV85	Membranipora membranacea	Syt1a	T7
  BV86	Membranipora membranacea	Syt1a	T7
  BV87	Membranipora membranacea	Syt1a	T7
  BV88	Membranipora membranacea	Syt1b	T7
  BV89	Membranipora membranacea	Syt1b	T7
  BV90	Membranipora membranacea	Syt1b	T7

   
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  Bruno Vellutini 11:56 on 2012/03/22 Permalink
  Tags: fez, foxg ( 3 ), Membranipora membranacea ( 140 ), nk ( 16 ), otp, pl10 ( 6 ), primers ( 7 ), synaptotagmin ( 5 )   

  Membranipora primers have arrived: Mm Syt1a, Syt1b, NK2.1, FoxG, Otp, PL10, Fez.