| Gene | Miniprep | Antisense Enzyme | ng/µL |
| Nano dlx | BV285 | SP6 | 159 |
| Ttra fz5-8 | BV288 | SP6 | 366 |
| Ttra fz7 | BV291 | SP6 | 2439 |
| Ttra dicer1b | BV162 | SP6 | 1867 |
| Ttra tudor1 | BV167 | SP6 | 1076 |
| Ttra argonaute | BV168 | SP6 | 323 |
Started probe PCR.
Began probe synthesis at 10:30.
Done with probe synthesis, see values above.
Setting up a pcr with gradient (63 +- 3 °C) at 12h:
| Gene | Primers Tm mean |
| Mm Six3/6 | 64.10 |
| Tt Dicer1b | 61.60 |
| Tt Runx | 63.15 |
| Tt Mael | 65.15 |
| Tt Piwia | 65.55 |
| Tt Tudor1 | 61.95 |
| Tt Ago | 62.95 |
| Tt PL10 | 63.10 |
| Tt Pum | 65.35 |
Extraction and ligation overnight at 4°C, except for MAEL.
Transformation and plating of colonies.
PL10 and Pumilio had very few colonies on the plate (insert numbers) and together with Piwia they did not produce any positive colony.
Put positive colonies to grow for minipreps.
| BV162 | Terebratalia transversa | Dicer1b |
| BV163 | Terebratalia transversa | Dicer1b |
| BV164 | Terebratalia transversa | Runx2 |
| BV165 | Terebratalia transversa | Runx2 |
| BV166 | Terebratalia transversa | Runx2 |
| BV167 | Terebratalia transversa | Tudor1 |
| BV168 | Terebratalia transversa | Argonaute |
| BV169 | Terebratalia transversa | Argonaute |
| BV170 | Terebratalia transversa | Argonaute |
| BV171 | Terebratalia transversa | NK6 |
| BV172 | Terebratalia transversa | NK6 |
| BV173 | Terebratalia transversa | NK6 |
| BV174 | Terebratalia transversa | Wnt7 |
| BV175 | Terebratalia transversa | Wnt7 |
| BV176 | Terebratalia transversa | Wnt7 |
Primers for more Terebratalia germline genes have arrived.