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Setting up a pcr with gradient (63 +- 3 °C) at 12h:
| Gene |
Primers Tm mean |
| Mm Six3/6 |
64.10 |
| Tt Dicer1b |
61.60 |
| Tt Runx |
63.15 |
| Tt Mael |
65.15 |
| Tt Piwia |
65.55 |
| Tt Tudor1 |
61.95 |
| Tt Ago |
62.95 |
| Tt PL10 |
63.10 |
| Tt Pum |
65.35 |
Extraction and ligation overnight at 4°C, except for MAEL.
24/07/12
Transformation and plating of colonies.
25/07/12
PL10 and Pumilio had very few colonies on the plate (insert numbers) and together with Piwia they did not produce any positive colony.
27/07/12
Put positive colonies to grow for minipreps.
| BV162 |
Terebratalia transversa |
Dicer1b |
| BV163 |
Terebratalia transversa |
Dicer1b |
| BV164 |
Terebratalia transversa |
Runx2 |
| BV165 |
Terebratalia transversa |
Runx2 |
| BV166 |
Terebratalia transversa |
Runx2 |
| BV167 |
Terebratalia transversa |
Tudor1 |
| BV168 |
Terebratalia transversa |
Argonaute |
| BV169 |
Terebratalia transversa |
Argonaute |
| BV170 |
Terebratalia transversa |
Argonaute |
| BV171 |
Terebratalia transversa |
NK6 |
| BV172 |
Terebratalia transversa |
NK6 |
| BV173 |
Terebratalia transversa |
NK6 |
| BV174 |
Terebratalia transversa |
Wnt7 |
| BV175 |
Terebratalia transversa |
Wnt7 |
| BV176 |
Terebratalia transversa |
Wnt7 |
