Setting up a pcr with gradient (63 +- 3 °C) at 12h:
Gene |
Primers Tm mean |
Mm Six3/6 |
64.10 |
Tt Dicer1b |
61.60 |
Tt Runx |
63.15 |
Tt Mael |
65.15 |
Tt Piwia |
65.55 |
Tt Tudor1 |
61.95 |
Tt Ago |
62.95 |
Tt PL10 |
63.10 |
Tt Pum |
65.35 |

Extraction and ligation overnight at 4°C, except for MAEL.
24/07/12
Transformation and plating of colonies.
25/07/12
PL10 and Pumilio had very few colonies on the plate (insert numbers) and together with Piwia they did not produce any positive colony.

27/07/12
Put positive colonies to grow for minipreps.
BV162 |
Terebratalia transversa |
Dicer1b |
BV163 |
Terebratalia transversa |
Dicer1b |
BV164 |
Terebratalia transversa |
Runx2 |
BV165 |
Terebratalia transversa |
Runx2 |
BV166 |
Terebratalia transversa |
Runx2 |
BV167 |
Terebratalia transversa |
Tudor1 |
BV168 |
Terebratalia transversa |
Argonaute |
BV169 |
Terebratalia transversa |
Argonaute |
BV170 |
Terebratalia transversa |
Argonaute |
BV171 |
Terebratalia transversa |
NK6 |
BV172 |
Terebratalia transversa |
NK6 |
BV173 |
Terebratalia transversa |
NK6 |
BV174 |
Terebratalia transversa |
Wnt7 |
BV175 |
Terebratalia transversa |
Wnt7 |
BV176 |
Terebratalia transversa |
Wnt7 |