Membranipora cloning final genes
Set PCR with:
Mm bmp2/4 | Mm otx1 | Mm chordin | Mm fgf8/17/18 |
Mm nanos | Mm piwi1 | Mm piwi2 | Mm vasa |
30/04/15
Ran gel:
Most important genes worked: bmp2/4, chordin and fgf8. piwi1 is bonus. Extract these and set a ligation for 3h. Transformed and plated around 18h.
01/05/15
Set colony PCR.
Put colonies to grow.
02/05/15
Extracted minipreps, set sequencing PCR and dropped at seq facility.
BV378 | Membranipora membranacea | Bmp2/4 | T7 | ok | + | SP6 |
BV379 | Membranipora membranacea | Bmp2/4 | T7 | ok | + | SP6 |
BV380 | Membranipora membranacea | Chordin | T7 | ok | + | SP6 |
BV381 | Membranipora membranacea | Chordin | T7 | ok | + | SP6 |
BV382 | Membranipora membranacea | Fgf8/17/18 | T7 | ok | + | SP6 |
BV383 | Membranipora membranacea | Fgf8/17/18 | T7 | ok | + | SP6 |
BV384 | Membranipora membranacea | Piwi1 | T7 | ok | + | SP6 |
BV385 | Membranipora membranacea | Piwi1 | T7 | ok | + | SP6 |