Membranipora simple staining

Just a simple staining of mixed stages of Membranipora using DAPI and Phalloidin 647 for morphology.

  • 3x 30min 0.2% PTx washes.
  • 1x PTx overnight at 4°C.

26/08/15

  • Staining with Phalloidin and DAPI for 2h.
  • Let embryos in PBS+DAPI 1:1000 at 4°C.

It failed… weird.