Membranipora simple staining
Just a simple staining of mixed stages of Membranipora using DAPI and Phalloidin 647 for morphology.
- 3x 30min 0.2% PTx washes.
- 1x PTx overnight at 4°C.
26/08/15
- Staining with Phalloidin and DAPI for 2h.
- Let embryos in PBS+DAPI 1:1000 at 4°C.
It failed… weird.