Novocrania wnt1 in situ with longer probe
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| Na wnt1 | Tt runx |
Did everything as usual and embryos looked normal.
13/05/15
Embryos quite damaged, including Terebratalia. Possible that pipette is not well calibrated?
Restarted in situ today with extreme gentleness. Added 5 µL protk in 10 mL (instead of 2.5 in 5 µL) using the P20 pipette. Fosfatase wash with 83 °C and added hybe buffer around 1800.
14/05/15
Add probe.