Novocrania wnt1 in situ with longer probe

Na wnt1 Tt runx

Did everything as usual and embryos looked normal.

13/05/15

Embryos quite damaged, including Terebratalia. Possible that pipette is not well calibrated?

Restarted in situ today with extreme gentleness. Added 5 µL protk in 10 mL (instead of 2.5 in 5 µL) using the P20 pipette. Fosfatase wash with 83 °C and added hybe buffer around 1800.

14/05/15

Add probe.