Novocrania wnt1 one more time

Set PCR with 60°C annealing, 1:45 elongation, 40 cycles and primers at 25mM.

wnt1 1:10 cDNA wnt1 1:20 cDNA
pax6 1:10 cDNA pax6 1:20 cDNA

07/05/15

2015-05-07 11.35.14

It worked. Extracted, ligated, transformed and plated.

08/05/15

Colony PCR and 2 volonies were fine:

2015-05-08 17.18.24

Put these to grow under the IDs:

T7
Na wnt1 long BV386
Na wnt1 long BV387

09/05/15

Extract miniprep and set sequencing reaction.