Membranipora cloning final genes

Set PCR with:

Mm bmp2/4 Mm otx1 Mm chordin Mm fgf8/17/18
Mm nanos Mm piwi1 Mm piwi2 Mm vasa

30/04/15

Ran gel:

2015-05-01 16.23.27

Most important genes worked: bmp2/4, chordin and fgf8. piwi1 is bonus. Extract these and set a ligation for 3h. Transformed and plated around 18h.

01/05/15

Set colony PCR.

2015-05-01 16.23.36

Put colonies to grow.

02/05/15

Extracted minipreps, set sequencing PCR and dropped at seq facility.

BV378 Membranipora membranacea Bmp2/4 T7 ok + SP6
BV379 Membranipora membranacea Bmp2/4 T7 ok + SP6
BV380 Membranipora membranacea Chordin T7 ok + SP6
BV381 Membranipora membranacea Chordin T7 ok + SP6
BV382 Membranipora membranacea Fgf8/17/18 T7 ok + SP6
BV383 Membranipora membranacea Fgf8/17/18 T7 ok + SP6
BV384 Membranipora membranacea Piwi1 T7 ok + SP6
BV385 Membranipora membranacea Piwi1 T7 ok + SP6