Probe synthesis Membranipora mesodermal genes

Set probe PCR for the first batch of genes published by the sequencing facility.

id species gene enzyme ng/µL comment
BV359 Membranipora membranacea FoxC SP6 1020
BV361 Membranipora membranacea FoxD SP6 445
BV362 Membranipora membranacea FoxF SP6  545
BV363 Membranipora membranacea Mef2 SP6  449
BV365 Membranipora membranacea Noggin2 SP6  988
BV367 Membranipora membranacea Eya SP6  826 short isoform
BV368 Membranipora membranacea Eya SP6  1271 long isoform
BV370 Membranipora membranacea mPRX SP6  620
BV372 Membranipora membranacea FHL SP6  321
BV374 Membranipora membranacea Pax6 SP6  1072
BV375 Membranipora membranacea Mox T7  1066
BV376 Membranipora membranacea Wnt4 SP6  1647

30/03/2015

Ran gel and extracted probe pcr.

2015-03-30 17.37.26

31/03/2015

Ran and extracted probe pcr for the second batch of sequences.

2015-03-31 12.19.30

2015-03-31 12.19.22

02/04/2015

Started riboprobe synthesis reaction. Ran for 6.5h and set to precipitate with 5µL of LiCl2.

03/04/2015

Precipitate and finish probes. Check above for values.