Immuno staining for azak and dmh1 treated Terebratalia


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Primary: Tyros. Tub. 1:500 (a594 mouse) / Synapsin II 1:500 (a647 rabbit).

Secondary: Alexa 594 (mouse) / Alexa 647 (rabbit) in a concentration of 1:200.

Staining: DAPI 1:500/ PHALL for 2h.

Mid blastula

1µM 10µM
control  dmh1 treated

Early gastrula

1µM 10µM
control  dhm1 control

Mid blastula to larva

1µM 10µM
control
  • Washed and cleaned embryos many times ~1h.
  • 1600 BSA 2x
  • 1730 NGS
  • 1830 Incubate

27/02/2015

  • 3x 5 min 0.2% PTx+BSA
  • 4x 30 min 0.2% PTx+BSA
  • 2h 0.2% PTx+NGS
  • Added A594 (mouse) and A647 (rabbit) 1:200.

28/02/2015

  • 3x 5 min 0.2% PTx+BSA
  • 4x 30 min 0.2% PTx+BSA
  • 2h staining in BODIPY FL and DAPI
  • 4x 5min 1x PBS

01/03/2015

Mounted in Murray and confocal-ed.