Azakenpaullone treated Terebratalia 


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Another Terebratalia in situ with Azakenpaullone treated embryos to check the localization of gene products. I want to see if the positioning of Pax6 and Pax2/5/8 has been affected by the displacement of the wnt pathway.

Blastula Mid Blastula Mid blastula to larva Early Gastrula Early Larva
1 µM 1 µM 1 µM 1 µM 1 µM
10 µM 10 µM 10 µM 10 µM 10 µM
C- C- C- C- C-

Proceeded normally with new 85 °C to remove fosfatases and incubated at 67°C.

12/02/15

Added probes.

14/02/15

Hybe washes went normally according to the protocol. Added Anti-DIG/AP at 2000.

15/02/15

Washed antibody many times with PBT 5x 15 min and 3x 30 min in PTw and let overnight at 4 °C around 1300.

16/02/15

Started developing at 0930. Signal is quite weak in the first 5 hours.