Cloning more segmentation genes


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I selected alternate primers for getting longer fragments and improving the quality of Novocrania in situs.

Nano ptc2, wnt1, smo1, smo2, fgf8, Mmem wnt4

2015-01-20 11.02.32

wnt1 did not work again, bad ptc2 again, smo1 and smo2 weakly worked, fgf8 worked, Mmem wnt4 not… Re-run Nano ptc2 with different primers:

2015-01-20 17.51.33

Combination of primers 3F and 2R (395 bp fragment) was the only one that worked. Transformed:

2015-01-23 17.16.01

T7
Na wnt5 BV352
Na wnt5 BV353
Na smo2 BV354
Na fgf8 BV355
Na fgf8 BV356
Na ptc2 BV357
Na ptc2 BV358