Cloning more segmentation genes
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I selected alternate primers for getting longer fragments and improving the quality of Novocrania in situs.
Nano ptc2, wnt1, smo1, smo2, fgf8, Mmem wnt4
wnt1 did not work again, bad ptc2 again, smo1 and smo2 weakly worked, fgf8 worked, Mmem wnt4 not… Re-run Nano ptc2 with different primers:
Combination of primers 3F and 2R (395 bp fragment) was the only one that worked. Transformed:
| T7 | |
| Na wnt5 | BV352 |
| Na wnt5 | BV353 |
| Na smo2 | BV354 |
| Na fgf8 | BV355 |
| Na fgf8 | BV356 |
| Na ptc2 | BV357 |
| Na ptc2 | BV358 |