Cloning Novocrania patched and longer clones of wnt1, wnt5

I made new primers for ptc2 and longer clones for wnt1 and wnt5. Set PCR overnight.

26/11/14

Ran gel and cut a band, but wnt1 and ptc2 did not work… OF COURSE! because fragment lengths are the following: wnt1=1239 bp, wnt5=1097 bp, ptc2=1258 bp! And the elongation time of the PCR was set to X.

2014-11-26 11.16.21

28/11/14

Re-tried the same PCR with a longer elongation time. The result was the same. Chema suggested running nested primers to see if something appears.

2014-11-28 17.18.15