Updates from November, 2014 Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 15:31 on 2014/11/25 Permalink
    Tags: , , ,   

    Cloning Novocrania patched and longer clones of wnt1, wnt5 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    I made new primers for ptc2 and longer clones for wnt1 and wnt5. Set PCR overnight.

    26/11/14

    Ran gel and cut a band, but wnt1 and ptc2 did not work… OF COURSE! because fragment lengths are the following: wnt1=1239 bp, wnt5=1097 bp, ptc2=1258 bp! And the elongation time of the PCR was set to X.

    2014-11-26 11.16.21

    28/11/14

    Re-tried the same PCR with a longer elongation time. The result was the same. Chema suggested running nested primers to see if something appears.

    2014-11-28 17.18.15

     
  • Bruno Vellutini 15:29 on 2014/11/25 Permalink
    Tags: , , ,   

    Probe synthesis Novocrania delta/notch 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Probe PCR set for Novocrania delta (BV348: Sp6) and notch (BV 350: Sp6).

    2014-11-25 16.44.03

    Cut and extracted.

    26/11/14

    Set transcription reaction for 7h. Added DNAse and 5 µL of lithium.

    27/11/14

    Finishing up.

    ng /µL
    Nano delta 1978
    Nano notch 1571
    Tt pou4 371

     

     
c
Compose new post
j
Next post/Next comment
k
Previous post/Previous comment
r
Reply
e
Edit
o
Show/Hide comments
t
Go to top
l
Go to login
h
Show/Hide help
shift + esc
Cancel