Transformation for remaining Terebratalia and Novocrania


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Terebratalia: ovo, serrate, pygopus, legless (from here)

Novocrania: notch, delta, gbx (from here)

Transformed and plated with 400 ┬ÁL.

19/11/14

Colony PCR.

2014-11-19 19.09.53

Set Nano delta and notch to grow.

20/11/14

Extracted minipreps for Nano notch and delta:

gene id seq primer antisense
Nano delta BV348 T7 SP6
Nano delta BV349 T7 SP6
Nano notch BV350 T7 SP6
Nano notch BV351 T7 T7

Set sequencing PCR for them.

24/11/14

Collected sequences. See orientation above.