Probe synthesis of Novocrania Hedgehog pathway
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Selected minipreps for probe synthesis of Novocrania Hedgehog pathway and remaining Terebratalia genes. From this post.
| BV326 | Terebratalia transversa | Lmx1 | T7 | ok | + | SP6 |
| BV328 | Terebratalia transversa | NK1a | T7 | ok | + | SP6 |
| BV330 | Terebratalia transversa | NK1b | T7 | ok | + | SP6 |
| BV332 | Novocrania anomala | Patched1 | T7 | ok | + | SP6 |
| BV336 | Novocrania anomala | Smoothened1 | T7 | ok | + | SP6 |
| BV338 | Novocrania anomala | Smoothened2 | T7 | ok | + | SP6 |
| BV340 | Novocrania anomala | Gli | T7 | ok | + | SP6 |
| BV343 | Novocrania anomala | Pax6 | T7 | ok | + | SP6 |
| BV345 | Novocrania anomala | Pax3/7 | T7 | ok | – | T7 |
| BV346 | Novocrania anomala | NK1 | T7 | ok | + | SP6 |
| TTR | Terebratalia | Pax6 | – | – | – | T7 DIG |
| TTR | Terebratalia | Pax6 | – | – | – | T7 DNP |
Set probe PCR to run overnight.
19/11/14
Ran gel and painfully extracted the PCR…
20/11/14
Started probe synthesis for the genes above at 9:30. Finished at 16:30 and precipitated overnight.
21/11/14
| gene | ng/µL | goal concentration | volume | total volume | volume of hybe buffer |
| Ttra lmx1 | 906 | 50 | 24 | 426 | 402 |
| Ttra nk1a | 974 | 50 | 24 | 458 | 434 |
| Ttra nk1b | 1098 | 50 | 24 | 516 | 492 |
| Nano ptc1 | 1075 | 50 | 24 | 505 | 482 |
| Nano smo1 | 2041 | 50 | 24 | 959 | 936 |
| Nano smo2 | 1830 | 50 | 24 | 860 | 836 |
| Nano gli | 488 | 50 | 24 | 229 | 206 |
| Nano pax6 | 1350 | 50 | 24 | 634 | 611 |
| Nano pax37 | 2224 | 50 | 24 | 1045 | 1022 |
| Nano nk1 | 1050 | 50 | 24 | 494 | 470 |
| Ttra pax6 dig | 1076 | 50 | 24 | 506 | 482 |
| Ttra pax6 dnp | 50 | 50 | 24 | 23 | 0 |