Transformation to sequencing of Novocrania
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I selected the 8 most important Novocrania genes from the first and second batched of cloning: ptc1, ptc2, smo1, smo2, gli, pax6, pax3/7, nk1
Transformed and plated.
12/11/14
Set colony PCR:
13/11/14
| BV332 | Novocrania anomala | Patched1 | T7 | ok | + | SP6 |
| BV333 | Novocrania anomala | Patched1 | T7 | ok | + | SP6 |
| BV334 | Novocrania anomala | Patched2 | T7 | ? | ||
| BV335 | Novocrania anomala | Patched2 | T7 | ? | ||
| BV336 | Novocrania anomala | Smoothened1 | T7 | ok | + | SP6 |
| BV337 | Novocrania anomala | Smoothened1 | T7 | ok | + | SP6 |
| BV338 | Novocrania anomala | Smoothened2 | T7 | ok | + | SP6 |
| BV339 | Novocrania anomala | Smoothened2 | T7 | ok | + | SP6 |
| BV340 | Novocrania anomala | Gli | T7 | ok | + | SP6 |
| BV341 | Novocrania anomala | Gli | T7 | ok | – | T7 |
| BV342 | Novocrania anomala | Pax6 | T7 | ok | – | T7 |
| BV343 | Novocrania anomala | Pax6 | T7 | ok | + | SP6 |
| BV344 | Novocrania anomala | Pax3/7 | T7 | ok | – | T7 |
| BV345 | Novocrania anomala | Pax3/7 | T7 | ok | – | T7 |
| BV346 | Novocrania anomala | NK1 | T7 | ok | + | SP6 |
| BV347 | Novocrania anomala | NK1 | T7 | ok | + | SP6 |