Transformation to sequencing of Novocrania

I selected the 8 most important Novocrania genes from the first and second batched of cloning: ptc1, ptc2, smo1, smo2, gli, pax6, pax3/7, nk1

Transformed and plated.

12/11/14

Set colony PCR:

2014-11-12 15.43.52

13/11/14

BV332 Novocrania anomala Patched1 T7 ok + SP6
BV333 Novocrania anomala Patched1 T7 ok + SP6
BV334 Novocrania anomala Patched2 T7 ?
BV335 Novocrania anomala Patched2 T7 ?
BV336 Novocrania anomala Smoothened1 T7 ok + SP6
BV337 Novocrania anomala Smoothened1 T7 ok + SP6
BV338 Novocrania anomala Smoothened2 T7 ok + SP6
BV339 Novocrania anomala Smoothened2 T7 ok + SP6
BV340 Novocrania anomala Gli T7 ok + SP6
BV341 Novocrania anomala Gli T7 ok – T7
BV342 Novocrania anomala Pax6 T7 ok – T7
BV343 Novocrania anomala Pax6 T7 ok + SP6
BV344 Novocrania anomala Pax3/7 T7 ok – T7
BV345 Novocrania anomala Pax3/7 T7 ok – T7
BV346 Novocrania anomala NK1 T7 ok + SP6
BV347 Novocrania anomala NK1 T7 ok + SP6