Membranipora U0126 inhibitor time windows

I set the U0126 inhibitor 10 µM with Membranipora at 15 °C. I am washing every 2 hours after 1h30 hours after activation as shown below (2x, for 24h and 48h fixation).

  • Activated: 1100
  • Inhibited: 1230
  • 1st wash: 1430
  • 2nd wash: 1630
  • 3rd wash: 1830
DMSO 3.5 hpa
5.5 hpa 7.5 hpa

 20/09/14

Fixed 24h.

21/09/14

Fix 48h.