Membranipora U0126 inhibitor time windows
I set the U0126 inhibitor 10 µM with Membranipora at 15 °C. I am washing every 2 hours after 1h30 hours after activation as shown below (2x, for 24h and 48h fixation).
- Activated: 1100
- Inhibited: 1230
- 1st wash: 1430
- 2nd wash: 1630
- 3rd wash: 1830
| DMSO | 3.5 hpa |
| 5.5 hpa | 7.5 hpa |
20/09/14
Fixed 24h.
21/09/14
Fix 48h.