Membranipora and U0126 inhibitor fine picked 


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Set up another U0126 inhibitor experiment. This time I’m using a fine picked and a coarse picked set of embryos. The setup is the same, but I picked only healthy 2-4 cell stage for the fine picked and a less strict sampling for the coarse picked (but still trying to fetch only good embryos.

FSW DMSO 5 µM 7.5 µM
1 µM 2.5 µM 10 µM 12.5 µM

Embryos were activated at 11h, picked from 12:30-13:30  and incubated at 13:30 (fine) and 14:00 (coarse) in the facility incubator at 15 °C!

19/06/14

Fixed after 24h.