Updates from June, 2014 Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 18:13 on 2014/06/16 Permalink
    Tags: ,   

    Another Membranipora 4D 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Started a Membranipora 4D from today’s spawning. Using eggs activated at 12h I have set the 4D at 15-16h. It is a vegetal/lateral view and the embryo looks quite stable.

    Filename: BCV_Mm_2014_06_16 (old Mmem_c16_06_14)

    17/06/14

    It failed during gastrulation.

     
  • Bruno Vellutini 17:26 on 2014/06/16 Permalink
    Tags: , ,   

    Another Membranipora U0126 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Another Membranipora spawning and I set up a new U0126 inhibitor experiment. I made 2 replicates again of the following setup:

    FSW DMSO 5 µM 7.5 µM
    1 µM 2.5 µM 10 µM 12.5 µM

    Embryos were activated at 12h and incubated at 15h in the facility incubator at 15 °C!

    I’ll fix the embryos on Tuesday after 24h.

    17/06/14

    Fixed after 24h.

     
  • Bruno Vellutini 12:32 on 2014/06/16 Permalink
    Tags:   


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Made 4 bowls completely full and stored the leaf back into the tank.

     
  • Bruno Vellutini 10:16 on 2014/06/16 Permalink
    Tags: , ,   

    Probe synthesis for Novocrania and Membranipora 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Starting probe synthesis (based on) for Novocrania en and pax258 with DNP utp for fluorescent in situ and many Membranipora genes.

    Probe ng/µL
    Nano en DNP 86
    Nano pax258 DNP 59
    Mmem dlx 378
    Mmem gbx 332
    Mmem pax258 1141
    Mmem wnt1 445
    Mmem wnt4b 64
    Mmem wnt5 167

    2014-06-16 18.12.39

    17/06/14

    Run probe synthesis reaction and started probe precipitation.

    18/06/14

    Nice pellets and specs are above.

     
c
Compose new post
j
Next post/Next comment
k
Previous post/Previous comment
r
Reply
e
Edit
o
Show/Hide comments
t
Go to top
l
Go to login
h
Show/Hide help
shift + esc
Cancel