Membranipora U0126 experiment with different concentrations


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With the nice Saturday spawning I managed to setup a new U0126 inhibitor experiment. I made 2 replicates of the following setup:

FSW DMSO 5 µM 7.5 µM
1 µM 2.5 µM 10 µM 12.5 µM

Embryos were activated at 14h and incubated at 17h in the facility incubator at 15 °C!

I’ll fix the embryos on Monday after 48h.

16/06/14

Replicate one everything was dead except for FSW control, very weird. The second replicate seemed fine, although there should be a lot of noise. I’ll repeat  the same today and fix 24h after.

I’ll fix with 48h.