Live membrane staining in Membranipora

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I decided to try the live membrane staining FM® 4-64FX, fixable analog of FM® 4-64 membrane stain with Membranipora embryos on the confocal. I dissolved the dessecated FM into 100 µL of DMSO and then diluted 2.5 µL in 500 µL of FSW (final concentration of 5µg/mL). I then added embryos to a slide and completed the volume with the staining solution and sealed as for the 4D. Using the 561 laser I get a really nice signal and I did a few xyzt stacks to test. They look nice!