Brachiopod in situ

In situ for additional Terebratalia frizzleds and repeating some Novocrania for better pictures.

Nano engrailed wnt1 wnt5 dlx pax258
Ttra frizzled 5/8 frizzled 7 dcr1b tdr1 ago

Started in situ with standard procedures. 8 min protk for Novocrania and 10 min for Terebratalia.

07/06/14

Added probes, embryos look good.

09/06/14

Washing day. Everything went as usual and put antibody around 18h.

10/06/14

Washed with PBT several times berween 10-15 min. Then PTw 30 min washes. Started developing.

Stopped Nano dlx after 2h30min because it was done, as well as Ttra fz5/8. The rest continued developing. fz7 got pinkish really fast

11/06/14

fz7 and ago got really dark. I don’t know if it is background or signal, even though it looks like there is real signal within the background. I stopped them as well as engrailed and pax258, which look better than the last in situ.

12/06/14

Stopped wnt1, wnt5 dcr1b, tdr1. Novocrania samples are looking dark ugly already…

13/06/14

Trying to clear out some of the background from fz7 and ago… letting in ethanol for many hours.