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  • Bruno Vellutini 00:12 on 2014/05/13 Permalink
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    Confocal with Membranipora propidium iodide and phalloidin far red 


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    Round of confocal with wild type Membranipora embryos. I mistakenly stained with propidium iodide instead of sytox green, but well. They were mounted in SlowFade to see if this is better than glycerol. However, it seems that it is incompatible with propidium and the nuclear staining became a general cytoplasm staining.. Many interesting scans of early and late gastrulation.

    MAX_Mmem pi-phallred.lif - Series012

     
  • Bruno Vellutini 00:06 on 2014/05/13 Permalink
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    Washed multiple times Lineus longissimus juveniles for RNA extraction. Put them into a RNA tube and wahsed and centrifuged more than 3 times with uvfiltered sea water. Then snapfreeze. They were around 40 days old.

     
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