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  • Bruno Vellutini 16:12 on 2014/05/08 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Washed Lineus longissimus larvae. Many larvae in the more advanced jar at 19 °C had already settled. I removed the swimming larvae by filtering the water until there was only a finger of water left. Then I rinsed the bottom to release the juveniles and transferred everything to a glass bowl.

    Juveniles look happy. I washed and cleaned the bowl as much as possible, but the juveniles stick to the bottom and to the pipets, so it is a bit hard. I transferred most of the worms to a clean bowl and kept the dirty as well in the incubator at 14 °C. I’ll separate many for RNAlater, a few for immunos and the rest to raise until adulthood.

    The other jar in fritzen room does not look so good. I’ll have a look at it next week. The jar from the incubator has many larvae, but they have the least advanced rudiment worm inside.

    I forgot to add food. I will add tomorrow.

     
  • Bruno Vellutini 10:11 on 2014/05/08 Permalink
    Tags: , , , , ,   

    Probe synthesis Ttra frizzleds Nano dlx and more 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77
    Gene Miniprep Antisense Enzyme ng/µL
    Nano dlx BV285 SP6  159
    Ttra fz5-8 BV288 SP6  366
    Ttra fz7 BV291 SP6  2439
    Ttra dicer1b BV162 SP6  1867
    Ttra tudor1 BV167 SP6  1076
    Ttra argonaute BV168 SP6  323

    Started probe PCR.

    2014-06-05 17.42.43

    09/05/14

    Began probe synthesis at 10:30.

    10/05/14

    Done with probe synthesis, see values above.

     
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