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  • Bruno Vellutini 23:33 on 2014/04/29 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Ran assembly with test data from MaSuRCA. It worked smoothly.

     
  • Bruno Vellutini 20:17 on 2014/04/29 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Washed and fed the larvae of Lineus longissimus. In the old jar the pilidium larvae have a well-formed juvenile with eyes, it will soon settle. In the second jar the juveniles are visible, but in a much earlier stage.

    20140429_210448

    I will let these metamorphose and fix this pilidium with well-developed juveniles after nice from the second jar.

     
  • Bruno Vellutini 20:10 on 2014/04/29 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Mounted and took some pictures of Membranipora in situs Chema did. Pattern looks sharp and with no background in the early stages. evx is restricted to one ectodermal cell during early gastrulation and 2 large lateral vegetal cells get activated later and it ends in the midgut and hindgut in early larva.

    20140429_192744

     
  • Bruno Vellutini 17:01 on 2014/04/29 Permalink
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    Cloning Ttra frizzled, Nano hox1, dlx, wnts 


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    Need to clone the remaining frizzleds from Terebratalia and wnts from Novocrania. Set overnight regular PCR with:

    Nano hox1 Nano wnt4 Nano wnt7 Nano dlx Ttra fz5/8 Ttra fz7

    30/04/14

    20140430_175703

    Unfortunately hox1 and the wnts did not work. I should change something in the PCR. I extracted dlx and frizzleds and set a ligation for 4h at RT.

    Transformed and plated the 3 genes and incubated at 37 °C.

    01/05/14

    Pic colonies for colony PCR. Run gel and set bacteria to grow.

    20140501_150448

    02/05/14

    Extracted minipreps and set sequencing PCR.

     
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