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Looking again at the wild caught cocoons from Lineus viridis I realized that, in fact, the eggs were not advanced blastula, but zygotes! Since then they haven’ t started cleaving! The egg mass that was laid on the day that we put then in the cold room had only one embryo at early cleavage, the rest were still 1 cell.

So, we don’t know the actual fertilization rate of wild cocoons! I put them back at 14 °C to see what happens. Hopefully they start cleaving.