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The girls went collecting Lineus viridis and found 4 cocoons in the rocks. They seem to be from L. viridis and not from the other similar species due to the amount of embryos per capsule. L. viridis has many, around 10-15, while the other species captured last year laid cocoons with 2-3 embryos per capsule.

Surprinsingly, all embryos withing each capsule seem to be healthy and developing! Fertilization in the field is much more efficient in the field. Our first guess is that the temperature is the problem. Water temperature is around 6 °C and we set our incubator to 14 °C to get the summer reproductive feeling and also because that is the temperature of the original description by von Döhren (10.1007/s10152-011-0266-z).

Our strategy then was to leave the incubator at 14 °C to keep the egg masses at the original temperature because we already know the developmental staging. We then moved the adult worms to the cold room where the temperature is 5 °C. There 2 tanks, old worms from the first collection and a few new worms from the last collection.

The goal is to wait them to lay a cocoon at this temperature and check for the fertilization rate. It might be higher!

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