Lineus longissimus spawned! 

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Lineus longissimus spawned today, probably early morning. Embryos were already at mid/late cleavage stage. We concentrated them and did some washes with UVFSW. I’ll start snap freezing for RNA extraction today and once per day. We need to get a transcriptome out of it.

Date Snap-freeze sample
1 02/04/14 0d Llon late cleavage
2 03/04/14 1d Llon blastula (date in the tube is wrong… says 04/04)
3 04/04/14 2d Llon late gastrula
4 05/04/14 3d Llon pilidium
5 07/04/14 5d Llon pilidium
6 12/05/14 2 month juvenile