Propidium+Phallacidin and Murray Clear for Novocrania engrailed fluorescent in situ

Since mounting with TDE was not so good (crystals and some weird noisy background) I am staining with propidium iodide and phallacidin and mounting with murray clear. This should give a clean embryo with the mesodermal signal visible enough for details. Fluorochrome for engrailed is Cy5.

17/12/13

Confocal.