Cloning Novocrania and Terebratalia again 


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Having the gene expression patterns of additional genes in Novocrania would be great to compare the body patterning with Terebratalia. I have selected some genes specific to apical, mantle, or pedicle lobe to try to map the expression of Terebratalia in Novocrania.

  • Apical lobe: wnt7, wnt8.
  • Mantle lobe: dlx, pax258, hox1.
  • Pedicle lobe: wnt4, wnt11.

Also a couple of wnt genes that hadn’t worked in Terebratalia are also being cloned. I forgot to include Nano dlx in the order :/

2013-11-26 21.29.18

27/11/13

Transformed and plated at 15h30. Put plates in incubator at 19h00.

28/11/13

Colony PCR and put cultures to grow. Wnt7 and Hox1 were not good so I plated them again with remaining bacteria.

2013-11-28 18.31.05

29/11/13

Miniprep and sequencing PCR. Dropped sequences in facility.

03/12/13

BV275 Terebratalia transversa Wnt2 T7 ok + SP6
BV276 Terebratalia transversa Wnt2 T7 ok + SP6
BV277 Terebratalia transversa Wnt2 T7 ok + SP6
BV278 Terebratalia transversa WntA T7 ok + SP6
BV279 Terebratalia transversa WntA T7 ok + SP6
BV280 Terebratalia transversa WntA T7 ok + SP6
BV281 Novocrania anomala Wnt7 T7 ok
BV282 Novocrania anomala Wnt11 T7 ok + SP6
BV283 Novocrania anomala Wnt11 T7 ok T7
BV284 Novocrania anomala Pax2/5/8 T7 ok + SP6