Re-synthesis of Novocrania wnt1 and wnt5 probes

Since the probes do not seem to be working for Novocrania in situ I will restart probe synthesis from scratch.

  • Set probe PCR.
  • Run Gel.
  • Extract.

2013-11-04 17.04.12

05/11/13

Transcription reaction for 6h and precipitate at -20 °C overnight.

06/11/13

Finished probe synthesis:

gene ng/µL
wnt1 3349
wnt5 185