Terebratalia additional double in situs


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Started Terebratalia double in situs with the following selected probes. Everything looks ok.

wnt1 (DNP) + wnt4 (DIG) en (DNP) + wnt7 (DIG)
wnt1 (DNP) + wnt8 (DIG) en (DNP) + pax258 (DIG)

22/10/13

  • Added probes to Terebratalia doubles.

24/10/13

  • Terebratalia doubles washing day.
  • Add Terebratalia first antibody anti-dig-pod 1:250.

25/10/13

  • Terebratalia doubles wash off first antibody.

27/10/13

  • Terebratalia doubles develop first probe and inactivate.
  • Terebratalia doubles incubate with second antibody.

28/10/13

  • Terebratalia doubles wash off second antibody.

29/10/13

  • Terebratalia doubles develop second probe.
  • Terebratalia doubles stain with phallacidin.
  • Terebratalia doubles embed in TDE+DAPI.

30/10/13

  • Mount Terebratalia doubles.

31/10/13

  • Confocal slot for Terebratalia doubles.

01/11/13

Here are some scans.