Updates from October, 2013 Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 16:29 on 2013/10/21 Permalink
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    Priapulus profiling in situ matching 


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    I got some in situ pictures from Priapulus and  tried to match them to the staged expression levels data. They are congruent more or less, although it is not so straightforward since stage pictures is not complete (early/late missing) and embryos are not exactly the specific rna stages.

    However, I can see gastrulation as a major burst of expression for most of the genes we have the in situs.

    bcatenin brachyury cdx evx fgf8 foxa1 foxq2 fz58 gata456 gsc

     
  • Bruno Vellutini 13:20 on 2013/10/21 Permalink
    Tags: , , , , , ,   

    Terebratalia additional double in situs 


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    Started Terebratalia double in situs with the following selected probes. Everything looks ok.

    wnt1 (DNP) + wnt4 (DIG) en (DNP) + wnt7 (DIG)
    wnt1 (DNP) + wnt8 (DIG) en (DNP) + pax258 (DIG)

    22/10/13

    • Added probes to Terebratalia doubles.

    24/10/13

    • Terebratalia doubles washing day.
    • Add Terebratalia first antibody anti-dig-pod 1:250.

    25/10/13

    • Terebratalia doubles wash off first antibody.

    27/10/13

    • Terebratalia doubles develop first probe and inactivate.
    • Terebratalia doubles incubate with second antibody.

    28/10/13

    • Terebratalia doubles wash off second antibody.

    29/10/13

    • Terebratalia doubles develop second probe.
    • Terebratalia doubles stain with phallacidin.
    • Terebratalia doubles embed in TDE+DAPI.

    30/10/13

    • Mount Terebratalia doubles.

    31/10/13

    • Confocal slot for Terebratalia doubles.

    01/11/13

    Here are some scans.

     
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