Isodiametra pulchra neuropeptide extraction

Extracted neuropeptides off the acoel Isodiametra pulchra using Gaspar Jekely protocol. Instead of using a mesh I gave a quick wash on distilled H2O, spin down the worms, then added 250 µL of extraction buffer and drilled them. After I added 350 µL more and continued with the protocol (20 min spin at 4°C). After spinning there was remnants of worms in the bottom, so I just took the clear supernatant and transferred to a new tube.