Fixing 32d Lineus viridis juveniles

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After failing to properly relax juveniles yesterday, I did the following.

  1. 45 min in MgCl2 in cold room
  2. 2 min in %2 cystein diluted in MgCl2
  3. 2 washes with MgCl2
  4. 15 min in MgCl2 in cold room
  5. Fix with ice cold 4% PFA in MgCl2

Standard fixation time ~3h in cold room + ~30 min at RT.