Lineus viridis in situ background 


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First in situs in Lineus viridis showed an epithelial background of what it looks like mucus cells:

Epidermis_insitu

To avoid this I’m fixing with a cystein treatment pre-fixation in juvenile stages (details later on). However, to be sure that the background cells are the mucus cells and that cystein will work I had a look at some basic descriptions. Below it is a DIC image of the epidermis with some gland cells.

Epidermis

Comparing this image with the in situ pictures it looks like the background cells are below the epidermis (dermis) and that these droplets in the epidermis are serous cells of protein content. A diagram from (10.1093/icb/25.1.37) seems to confirm this for heteronemerteans:

Norenburg1985_integument

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