Updates from April, 2013 Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 16:44 on 2013/04/09 Permalink

    Lineus red (previously known as Lineus ruber) laid an egg mass that looks healthy on the 08/04/13. I checked today (1 day after) and it seems that one embryo out of +10 of each egg bundle is cleaving. I will follow up this egg mass to see what happens.


  • Bruno Vellutini 16:06 on 2013/04/06 Permalink

    Yesterday I tried dissecting another Lineus sp. egg mass with gastrulae, but I basically failed. The jelly was too sticky and hard to get rid off. I did a 10 min step in 5% Cystein to see what happened and the jelly became more elastic, but still hard to work on. I then left the dish shaking in the cold room overnight.

    Today I found out that the whole jelly became flakes and the egg bundles were loose, ready to be dissected. Pretty cool. The embryos looked ok, but I’m not sure they were still developing. I will try this again.

    2013-04-06 16.37.01 2013-04-06 16.38.29

  • Bruno Vellutini 12:59 on 2013/04/05 Permalink
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    Put 1 dish of L. longissimus in RNALater. First I washed the dish twice, then picked the larvae to a new clean smaller dish with UVFSW, and finally I picked the larvae to put in RNALater with a P10 (works best because you don’t get much water).

  • Bruno Vellutini 17:04 on 2013/04/04 Permalink
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    Fixing ~50 gastrula stage embryos of a egg mass from the left aquarium, the one with the green Lineus (+pink eyed nemertines). Egg mass looks clearer and with fewer eggs per case (1-3, previous Lineus red had ~9 embryos per case). Fixation protocol for in situ.

    I have also cleaned the 3 Lineus longissimus dishes many times. Tomorrow I start putting them in RNALater.

  • Bruno Vellutini 16:53 on 2013/04/03 Permalink
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    Agalma Terebratalia transcriptome 

    Final step to compare different assemblies of RNA-Seq was done on 01/04/2013. I’m now compiling the results into tables to define the best alternative. Results are in the wiki.

  • Bruno Vellutini 14:00 on 2013/04/02 Permalink
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    Easter eggs! 

    First day after Easter holidays I found several egg cases in the Lineus red and green aquariums. They were sorted by aquarium and appearance and kept in the incubator in small plastic dishes. Lineus longissimus also spawned! Maybe on Saturday or Sunday? There was a huge white mass of eggs/sperm in the water. I collected everything in glass bowls, did a few changes using a filter and started picking embryos that were swimming (gastrula and early pilidium larvae).

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