Cystein treatment for 8d Lineus red egg mass from 14/04/13. 5 minutes in %5 cystein in FSW inside cold room. Dish washed twice with FSW and let to rock in the cold room overnight.


Egg bundles were perfectly dissociated. I just needed to pipet a bit to release some bundles. I transfer some to a new dish and began to peel each bundle. It took me 1h30 for 1 egg mass. I fixed the embryos for 2h in the cold room + 1h at room temperature. 60% fixed for in situ and 40% for confocal.