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  • Bruno Vellutini 15:39 on 2013/04/17 Permalink
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    Meara miniprep check 


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    Ran a PCR to check the miniprep swap I did last time. I selected 2 sequences that will be used for in situ probes and ran a PCR with primers from both genes (boule and gus a). The correct gene should be amplified by its primer pairs and confirm the identity of the miniprep. As it can be seen in the gel I did indeed swap the labels of the miniprep, so BV226 is gus a and BV229 is boule.

    2013-04-17 15.10.07

     
  • Bruno Vellutini 13:51 on 2013/04/17 Permalink
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    Picked last dish of Lineus longissimus larvae with ~3 weeks old to RNALater.

     
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