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  • Bruno Vellutini 18:24 on 2013/03/25 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Starting a Terebratalia RNAseq CLC assembly with the following parameters:

    Automatic word size = ON
    Automatic bubble size = ON
    Minimum contig length = 170
    Auto-detect paired distances = ON
    Perform scaffolding = OFF
    
    Map reads back = ON
    mismatch = 2
    insertion = 3
    deletion = 3
    length = 0.5
    similarity = 0.8
    Colorspace alignment = ON
    Global alignment = OFF
    Update contigs = ON
    Create list of un-mapped = OFF
    
    Create report = ON
    Result handling = Save
    Make log = ON
    
     
  • Bruno Vellutini 17:10 on 2013/03/25 Permalink
    Tags: , , , , , , ,   

    Cloning of Terebratalia wnt2 and other Meara RNA binding proteins 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Started a PCR with Terebratalia wnta, wnt2; and Meara ago a, ago b, ago c, bru a, bru b, mago, mex3, orb2.

    2013-03-26 11.47.59 2013-03-28 15.23.26

    12/04/13

    Put colonies to grow.

    Colony T7
    Ago a c1 BV235
    Ago a c2 BV236
    Ago a c3 BV237
    Ago b c1 BV238
    Ago b c2 BV239
    Ago b c4 BV240
    Bru a c1 BV241
    Bru a c2 BV242
    Bru a c3 BV243
    Mago c1 BV244
    Mago c2 BV245
    Mago c3 BV246
    Orb2 c1 BV247

     
  • Bruno Vellutini 11:09 on 2013/03/25 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    After last Lineus collection I rearranged the worms collected a year ago to a small glass bowl. Some time in the last week they laid two egg cases which I found today. I transferred the two egg cases to a small glass bowl with clean sea water.

    So apparently we can keep these worms in the facility with irregular and scarce food supply and they still manage to lay eggs.

    Recently collected worms did not lay anything yet.

     
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