Fluorescent in situ of Terebratalia engrailed

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Mounted a slide with Terebratalia embryos from the first fluorescent/double in situ I tried for the segmentation genes and observed under the confocal. Mounting media had DAPI (magenta) and en is green. Although there is background it is possible to distinguish the real signal.

Here are some max-projections to illustrate:

MAX_Ttra En_DNP + DAPI.lif - Series031 MAX_Ttra En_DNP + DAPI.lif - Series028 MAX_Ttra En_DNP + DAPI.lif - Series031